Medical knowledge

With the current state of medical science, osteoarthritis cannot be cured or completely stopped. The contemporary therapies focus mainly on the use of blood replacement products (PRP, IRAP), non-steroidal anti-inflammatory drugs (NSAIDs), or introduce dietary and rehabilitation strategies. In extreme cases, palliative surgical techniques are also employed.

It needs, however, to be stressed that in four-legged patients, a large number of therapy-resistant cases are present, and negative side-effects of medical procedures commonly occur. With this in mind, the veterinary community watches studies over new OA treatment methods with hope, among them the method involving the use of mesenchymal stem cells (MSC) in the treatment of this disorder.

Laboratory studies have shown that the use of MSC in the treatment of osteoarthritis results in marked reduction of inflammation, which leads to evident alleviation of pain and contributes to natural processes of reconstruction of the damaged joint tissues… AND HERE STEM4CELLS COMPANY COMES with its innovative method of cells production, preservation method for cells’ longevity and application maneuvers.

Scientific publications and real life experience

  • Villatoro AJ, Fernández V, Claros S, Rico-Llanos GA, Becerra J, Andrades JA. Use of adipose-derived mesenchymal stem cells in keratoconjunctivitis sicca in a canine model. Biomed Res Int. 2015;2015:527926. doi: 10.1155/2015/527926. Epub 2015 Feb 23. PMID: 25802852; PMCID: PMC4352730. [PDF]
  • Villatoro AJ, Fernández V, Claros S, Alcoholado C, Cifuentes M, Merayo-Lloves J, Andrades JA, Becerra J. Regenerative Therapies in Dry Eye Disease: From Growth Factors to Cell Therapy. Int J Mol Sci. 2017 Oct 28;18(11):2264. doi: 10.3390/ijms18112264. PMID: 29143779; PMCID: PMC5713234. [PDF]
  • Villatoro AJ, Hermida-Prieto M, Fernández V, Fariñas F, Alcoholado C, Rodríguez-García MI, Mariñas-Pardo L, Becerra J. Allogeneic adipose-derived mesenchymal stem cell therapy in dogs with refractory atopic dermatitis: clinical efficacy and safety. Vet Rec. 2018 Dec 1;183(21):654. doi: 10.1136/vr.104867. Epub 2018 Aug 29. PMID: 30158120. [PDF]
  • Villatoro AJ, Claros S, Fernández V, Alcoholado C, Fariñas F, Moreno A, Becerra J, Andrades JA. Safety and efficacy of the mesenchymal stem cell in feline eosinophilic keratitis treatment. BMC Vet Res. 2018 Mar 27;14(1):116. doi: 10.1186/s12917-018-1413-4. PMID: 29587744; PMCID: PMC5870249. [PDF]
  • Villatoro AJ, Alcoholado C, Martín-Astorga MC, Fernández V, Cifuentes M, Becerra J. Comparative analysis and characterization of soluble factors and exosomes from cultured adipose tissue and bone marrow mesenchymal stem cells in canine species. Vet Immunol Immunopathol. 2019 Feb;208:6-15. doi: 10.1016/j.vetimm.2018.12.003. Epub 2018 Dec 18. PMID: 30712794. [PDF]
  • Villatoro AJ, Martín-Astorga MDC, Alcoholado C, Becerra J. Canine colostrum exosomes: characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity. BMC Vet Res. 2020 Nov 2;16(1):417. doi: 10.1186/s12917-020-02623-w. PMID: 33138803; PMCID: PMC7607682. [PDF]
  • Villatoro AJ, Alcoholado C, Martín-Astorga MDC, Rico G, Fernández V, Becerra J. Characterization of the secretory profile and exosomes of limbal stem cells in the canine species. PLoS One. 2020 Dec 29;15(12):e0244327. doi: 10.1371/journal.pone.0244327. PMID: 33373367; PMCID: PMC7771867. [PDF]
  • Villatoro AJ, Martín-Astorga MDC, Alcoholado C, Sánchez-Martín MDM, Becerra J. Proteomic Analysis of the Secretome and Exosomes of Feline Adipose-Derived Mesenchymal Stem Cells. Animals (Basel). 2021 Jan 24;11(2):295. doi: 10.3390/ani11020295. PMID: 33498940; PMCID: PMC7912403. [PDF]
  • Villatoro AJ, Martín-Astorga MDC, Alcoholado C, Cárdenas C, Fariñas F, Becerra J, Visser R. Altered Proteomic Profile of Adipose Tissue-Derived Mesenchymal Stem Cell Exosomes from Cats with Severe Chronic Gingivostomatitis. Animals (Basel). 2021 Aug 23;11(8):2466. doi: 10.3390/ani11082466. PMID: 34438923; PMCID: PMC8388770. [PDF]
  • Monferrer E, Sanegre S, Vieco-Martí I, López-Carrasco A, Fariñas F, Villatoro A, Abanades S, Mañes S, de la Cruz-Merino L, Noguera R, Álvaro Naranjo T. Immunometabolism Modulation in Therapy. Biomedicines. 2021 Jul 9;9(7):798. doi: 10.3390/biomedicines9070798. PMID: 34356862; PMCID: PMC8301471. [PDF]
  • Villatoro AJ, Martín-Astorga MDC, Alcoholado C, Kazantseva L, Cárdenas C, Fariñas F, Becerra J, Visser R. Secretory Profile of Adipose-Tissue-Derived Mesenchymal Stem Cells from Cats with Calicivirus-Positive Severe Chronic
    Gingivostomatitis. Viruses. 2022 May 25;14(6):1146. doi: 10.3390/v14061146. PMID: 35746618; PMCID: PMC9228153. [PDF]
  • Villatoro AJ, Alcoholado C, Martín-Astorga MDC, Rubio N, Blanco J, Garrido CP, Becerra J. Suicide gene therapy by canine mesenchymal stem cell transduced with thymidine kinase in a u-87 glioblastoma murine model: Secretory profile and antitumor activity. PLoS One. 2022 Feb 15;17(2):e0264001. doi: 10.1371/journal.pone.0264001. PMID: 35167620; PMCID: PMC8846542. [PDF]